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. 2019 Jun 18;10:180. doi: 10.1186/s13287-019-1285-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — miR-34a overexpression enhanced the osteoblastic differentiation of 2 Gy irradiated BMSCs in vitro. a ALP staining, intracellular ALP activity, Alizarin red staining, and the quantitative colorimetric results of Alizarin red staining for BMSCs irradiated with 2 Gy after osteogenic induction; scale bar = 1 cm. b Gene expression of Runx2, Alp, Col-1, and Ocn after 14 days of osteogenic induction. c Western blot analysis of RUNX2, ALP, COL-1, OCN, and GAPDH after 14 days of osteogenic induction. d The quantitative analysis of the Western blot results relative to GAPDH (fold to control). Data are shown as mean ± SD, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001