Fig. 1.

Immunohistochemical expression of FAM83H and β-catenin in human osteosarcoma tissue and statistical analysis to determine cut-off values. a The expression of FAM83H protein by western blot in normal osteoblast cells and U2OS, MG63, and KHOS/NP osteosarcoma cells. b Immunohistochemical expression of FAM83H and β-catenin in human osteosarcomas. Original magnification, × 400. c Receiver operating characteristic curve of cytoplasmic and nuclear FAM83H expression for death from osteosarcoma. The cut-off points for the cytoplasmic expression of FAM83H (Cy-FAM83H), nuclear expression of FAM83H (Nu-FAM83H), and β-catenin expression were selected at the highest positive likelihood ratio points for the estimation of death in osteosarcoma patients with receiver operating characteristic curve analysis. The red arrow, blue arrowhead, and green empty arrowhead indicate cut-off points for the expression of Cy-FAM83H, Nu-FAM83H, and β-catenin, respectively. AUC; area under the curve