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. 2019 Jun 18;38:267. doi: 10.1186/s13046-019-1274-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — The effects of FAM83H expression on proliferation and invasiveness in osteosarcoma cells. The MTT (a) and colony forming assay (b) was performed after knock-down of FAM83H with shRNA for FAM83H #1 and #2 or overexpression of FAM83H in the U2OS, MG63, and KHOS/NP cells. The knock-down and overexpression of FAM83H after transfection is presented with western blot for FAM83H. The migration (b) and invasion activity (c) was performed after knock-down of FAM83H or overexpression of FAM83H in the U2OS and MG63 cells. CV; control vector, OE; overexpression, *; versus control vector transfection; P < 0.05, **; versus control vector transfection; P < 0.001