Table 1.
Primer Sequences Used for Quantitative Real-time Polymerase Chain Reaction
| Gene | Primer Sequence | Product size | Accession number | |
|---|---|---|---|---|
| FAM83H | forward | CATGGTCCAGACAACCTGTG | 214 | NM_198488.3 |
| reverse | GCTGGATACCAGGAGGACAA | |||
| CTNNB1 (β-catenin) | forward | AAAATGGCAGTGCGTTTAG | 100 | NM_001904.3 |
| reverse | TTTGAAGGCAGTCTGTCGTA | |||
| CCND1 (Cyclin D1) | forward | GAGGAAGAGGAGGAGGAGGA | 236 | NM_053056.2 |
| reverse | GAGATGGAAGGGGGAAAGAG | |||
| p27 | forward | TCTACTGCGTGGCTTGTCAG | 240 | AB001740.1 |
| reverse | CTGTATTTGGAGGCACAGCA | |||
| Vimentin | forward | GAGAACTTTGCCGTTGAAGC | 170 | NM_003380.3 |
| reverse | TCCAGCAGCTTCCTGTAGGT | |||
| SNAL1 (Snail) | forward | ACCCCACATCCTTCTCACTG | 217 | NM_005985.3 |
| reverse | TACAAAAACCCACGCAGACA | |||
| GAPDH | forward | AACAGCGACACCCACTCCTC | 258 | NM_001256799.1 |
| reverse | GGAGGGGAGATTCAGTGTGGT | |||
Web link to accession numbers: https://www.ncbi.nlm.nih.gov/gene