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. 2019 Jun 18;10:179. doi: 10.1186/s13287-019-1272-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Phenotype and multidirectional differentiation potential of En-PSCs. a Immunofluorescence staining in human endometrium displayed CD146, CD31, PDGFRβ, and α-SMA. The arrow showed En-PSCs. Scale bars, 25 μm. b En-PSCs (CD146+CD34-CD45-CD56-CD144-) were sorted by flow cytometry. c–m Flow cytometry analysis of immune-markers in En-PSCs. n–p En-PSCs expressed PDGFRβ, NG2, and α-SMA. Scale bars, 25 μm. q Intracellular lipid droplet induced from En-PSCs were detected by Oil red O staining. Scale bars, 50 μm. r Calcified nodule stained with Alizarin red S indicated that En-PSCs differentiated to osteogenic lineage. Scale bars, 50 μm. Neural-like differentiation was confirmed by immunofluorescence staining with anti-NSE antibody (s) and anti-NF-M antibody (t). Scale bars, 50 μm