Fig. 2.

En-PSCs with elevated CYR61 expression induce angiogenesis. a The tube formation after treatment with CYR61 alone, combined CYR61&Anti-CYR61, VEGF alone, were microscopically compared. b The mean number of observed tubes was counted in three wells, with three to five random fields per well. c ELISA were performed 48 h after transfection of si-CYR61 or ov-CYR61. The concentration of CYR61 was measured in the supernatant of transfected En-PSCs. d Tube formation assays were performed 48 h after transfection of si-CYR61 or ov-CYR61. The tube formation after co-culture of HUVECs with transfected En-PSCs were microscopically compared. f The mean number of observed tubes was counted in three wells, with three to five random fields per well. Scale bars, 200 μm. e Effect of transfected En-PSCs on HUVECs migration. The HUVECs migrated to the bottom of the transwell membrane were stained with crystal violet. Scale bars, 500 μm. Local magnification of figures were showed in the pictures below. Scale bar, 50 μm. g The mean number of observed cells was then counted per well, with five random fields per well (magnification of × 400). Each experiment was repeated three times. Data were presented as mean + SEM. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001