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. 2019 Jun 12;9:192. doi: 10.3389/fcimb.2019.00192

Figure 3.

Figure 3

IDO1 and IDO2 mRNA expression measurements by RNA-Seq and qPCR. Integrative Genomics Viewer (Thorvaldsdóttir et al., 2013) images showing the coverage of IDO1 (A) and IDO2 (B) genes by sequencing reads in a representative C. muridarum infected and uninfected control samples. IDO1 and IDO2 gene expression inductions measured by qPCR in C. muridarum and C. pneumoniae infected lung tissues (n = 3) (C). IDO1 and IDO2 gene expression fold changes in each infected mice (n = 3) were calculated by comparing the average IDO1 and IDO2 expressions in the uninfected controls (n = 3). IDO1 and IDO2 gene expressions were normalized by the beta-actin gene expressions. ΔCt values (CtIDO-Ctactin) of the infected and uninfected samples was compared by Student's t-test. **P < 0.01 *P < 0.05.