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. 2019 Jun 11;25:4362–4369. doi: 10.12659/MSM.914579

Figure 3.

Figure 3

Effects of miR-30c-5p on the expression of CD86, CD206, iNOS, and IL-10 in THP-1 cells. HTP-1 cells were induced with PMA. Cells were treated with miR-30c-5p agomir and miR-30c-5p antagomir for 3 hours, then the cells were cultured normally for 48 hours. The mRNA levels of miR-30c-5p, CD86, CD206, iNOS, and IL-10 were determined with qRT-PCR (A–E). The protein levels of CD86, CD206, iNOS, and IL-10 were detected with western blot (F–J). miR-30=miR-30c-5p. n=3. The values were presented as mean ±SD, and the data were analyzed by ANOVA with Turkey’s multiple test (a versus Control group; a=b=c P<0.05, aa=bb=cc P<0.01). IL – interleukin; iNOS – inducible nitric oxide synthase; PMA – phorbol myristate acetate; qRT-PCR – quantitative reverse transcription-polymerase chain reaction; SD – standard deviation; ANOVA – analysis of variance.