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. 2019 Jun 10;25:4314–4321. doi: 10.12659/MSM.916881

Figure 1.

Figure 1

microRNA-378a (miR-378a) regulates reactive oxygen species (ROS) in human lens epithelial cells (HLECs). (A) Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis shows the level of microRNA-378a (miR-378a) in human lens epithelial cells (HLECs) transfected with NC, miR-378a mimic, and miR-378a inhibitor. All data are presented as the mean ±SD. The two-tailed unpaired Student’s t-test was performed for comparison of the two groups. (*** P<0.001). (B) RT-qPCR analysis shows the mRNA level of the SOD1 gene in HLEC cells transfected with NC, miR-378a mimic, and miR-378a inhibitor. All data are presented as the mean ±SD. The two-tailed unpaired Student’s t-test was performed for comparison of the two groups. (** P<0.01). (C, D) The levels of the ratio of ethidium to dihydroethidium (E: DHE) for superoxide (O2−), and 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (C-H2DCFDA) fluorescence for hydrogen peroxide (H2O2) in HLEC cells transfected with NC, miR-378a mimic, and miR-378a inhibitor. All data are presented as the mean ± SD. The two-tailed unpaired Student’s t-test was performed for comparison of the two groups. (* P<0.05).