Table 1. Cytotoxicity of M-TKs in vitro and in vivo. IC₅₀ values on seven cell lines (48 hours, μM) and LD₅₀ values on zebrafish embryos (10–24 hpf, μM) for Cu-TK, Zn-TK, Fe-TK, Mn-TK Cd-TK, TK and cisplatin. All assays were conducted in triplicate and the mean IC₅₀ ± standard deviation was determined.

	Cell lines							Zebrafish embryo
	HeLa	A2780	A2780/cis	MDAMB	PC3	MCF-7	HEK-293
Cu-TK	13.3 ± 0.1	3.2 ± 0.6	1.3 ± 0.1	2.4 ± 0.3	27.7 ± 1.9	4.8 ± 0.3	20.4 ± 0.5	4
Zn-TK	5.4 ± 0.5	8.3 ± 0.7	5.7 ± 1.0	6.0 ± 0.5	44.5 ± 3.2	17.7 ± 0.2	11.8 ± 0.2	8.8
Fe-TK	1.3 ± 0.6	5.2 ± 0.8	2.3 ± 1.2	3.1 ± 0.2	0.9 ± 0.1	9.1 ± 0.7	≫100	8
Cd-TK	1.5 ± 0.2	2.1 ± 0.3	0.6 ± 0.2	1.9 ± 0.1	11.7 ± 1.8	7.8 ± 2.5	16.8 ± 0.1	8
Mn-TK	3.3 ± 0.2	4.1 ± 0.1	4.2 ± 0.2	0.8 ± 0.05	7.5 ± 0.2	3.4 ± 0.2	24.3 ± 0.7	4.8
TK	≫100	≫100	54.6 ± 1.4	≫100	≫100	27.4 ± 0.3	≫100	≫100
Cisplatin	25.7 ± 4.3	11.2 ± 0.2	28.1 ± 1.0	16.5 ± 0.6	15.4 ± 1.3	5.8 ± 2.8	1.7 ± 0.5	250