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. 2019 Jun 4;12:145. doi: 10.3389/fnmol.2019.00145

FIGURE 2.

FIGURE 2

Expression of Scn2a mRNA in both glutamatergic and GABAergic neurons. (A,B) Expression of Scn2a mRNAs in Vglut1/2-positive glutamatergic neurons (A) and Gad1/2-positive GABAergic neurons (B) in the neocortex and hippocampus in the mouse brain (P56), as detected by fluorescence in situ hybridization. Coronal brain sections were triply stained for Scn2a, Vglut1/2 or Gad1/2, and DAPI (nuclear stain; blue). Images at right show enlarged views of white boxes in the images at left. Arrowheads indicate neurons that express both Scn2a and neuronal markers; cells indicated by yellow arrowheads were further enlarged to highlight coexpression of the markers. Scale bar, 20 and 10 μm for left and right scale bars, respectively, in each row. (C–E) Expression of Scn2a mRNA in PV-, SST-, or VIP-expressing GABAergic neurons in the neocortex and hippocampus in the mouse brain (P56), as detected by fluorescence in situ hybridization. Scale bar, 20 and 10 μm for left and right scale bars, respectively, in each row.