Skip to main content
. 2019 Jun 4;12:145. doi: 10.3389/fnmol.2019.00145

FIGURE 5.

FIGURE 5

Scn2a+/- mice show impaired spatial learning and memory and enhanced long-term fear memory. (A–C) Impaired spatial learning and memory in both the initial and reversal phases of the Morris water-maze test in Scn2a+/- mice (3–4 months), as shown by escape latency, time spent in quadrant, and number of exact platform crossings in the learning phase (day 1–7), reversal phase (day 9–11), and respective probe tests (days 8 and 12). Data are presented as means ± SEM. n = 23 mice for WT and 28 for HT, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ns, not significant, two-way ANOVA with Sidak’s multiple comparison test, Mann–Whitney test, and Student’s t-test. (D) Normal behavior of Scn2a+/- mice (2–3 months) in novel object-recognition test, as shown by novel-object preference. Note object exploration times are normal on both first and second days. n = 16 mice for WT and 18 for HT, ns, not significant, Student’s t-test. (E) Normal contextual fear memory acquisition and 24-h memory in Scn2a+/- mice (2–3 months), but enhanced 7-day fear memory in contextual fear-conditioning tests, as shown by freezing levels. Note that the 7-day experiment was performed directly after fear memory acquisition (no 24-h retrieval experiment). n = 13 mice for WT and 10 for HT, ∗∗P < 0.01, ns, not significant, Mann–Whitney test and Student’s t-test. (F) Normal motor learning of Scn2a+/- mice (3–4 months) in the rotarod test, as shown by latency to fall from the rotating rod. n = 8 mice for WT and 12 for HT, two-way ANOVA with Sidak’s multiple comparison test.