Figure 5.

Cloning efficiency of rocking melanoma cells exposed to esomeprazole or everolimus. (a) Representative images (left) of clonogenic efficacy of acidic and nonacidic A375M6 melanoma cells after exposure to 48h of rocking condition in the presence or in the absence of 100 μM esomeprazole (a proton-pump inhibitor). After treatment, the cells were seeded in 10 mm plates (5000 cells/dishes, in triplicate) and incubated for 15 days. Quantification (right) of cloning efficiency expressed as number of colonies (untreated, UT). (b) Representative images (left) of clonogenic efficacy of acidic and nonacidic A375M6 melanoma cells after exposure to 48h of rocking condition in the presence or in the absence of 2 μM everolimus (an inhibitor of mTOR). After treatment, the cells were seeded in 100 mm plates (5000 cells/dishes, in triplicate) and incubated for 15 days. Quantification (right) of cloning efficiency expressed as number of colonies (untreated, UT). Data are expressed as mean ± SEM of at least three independent experiments. ∗ p<0.05.