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. 2019 Jun 2;2019:2715968. doi: 10.1155/2019/2715968

Figure 3.

Figure 3

Detection and determination of FZD-10 expression levels in sEVs isolated from cancer patients with metastasis by Western blotting and densitometry analysis. Representative Western blotting of FZD-10 and three exosomal/EV protein markers (Hsp70, CD-63, and ALIX) in sEVs extracted from patients with metastatic GC (Px GC) and metastatic CRC (Px CRC), before surgery and at different treatment steps. Molecular mass markers indicated on the right. The same load (20 μg) of samples based on total protein content (a). Semiquantitative evaluation of relative FZD-10 expression in sEVs extracts by densitometry analysis of protein bands in (a). FZD-10 bands were measured and normalized with corresponding housekeeping Hsp70 bands, for each CRC and GC patient (three replicates). Average value of FZD-10 expression levels among all CRC or GC or metastastic CRC patients reported in graph. (∗) p<0.005 versus healthy control. n= 6 for CRC, n=6 for GC, and n=8 for healthy donors (b).