Table 1. Results for validation parameters (Dried pea).
Nicotinic acid | Nicotinamide | |
---|---|---|
Linear regression equation | y = 371,678x − 106,410 | y = 649,469x + 33,576 |
r2 | 0.9979 | 0.9978 |
Range of linearity (μg/mL) | 1–10 | 1–10 |
Repeatability (RSD, n = 10) | 2.0% | 2.0% |
Reproducibility (RSD of intra-day studies, n = 16) | 2.08% | 2.04% |
LOD (μg/mL) | 0.005 | 0.004 |
LOQ (μg/mL) | 0.018 | 0.013 |
Accuracy (a, recovery; b, RSD; n = 10) | 104.2%a, 0.34%b | 96.4%a, 0.28%b |
x, amount (μg/mL); y, peak area; r2, correlation coefficient;
LOD, limit of detection; LOQ, limit of quantification; RSD, relative standard deviation.