Antibodies |
Chicken anti-GFP |
Abcam |
Cat# ab13970; RRID: AB_300798 |
Rabbit anti-NeuN |
Cell Signaling |
Cat# 12943; RRID: AB_2630395 |
Mouse anti-mCherry |
Saint John’s |
Cat#STJ97087; RRID:AB_2687534 |
Rabbit anti-DsRed |
Clontech Laboratories |
Cat# 632496; RRID:AB_10013483 |
Rabbit anti-S100b |
Abcam |
Cat# ab41548; RRID: AB_956280 |
Guinea pig anti-Neun |
Synaptic Systems |
Cat# 266 004; RRID:AB_2619988 |
Guinea pig anti-cFOS |
Synaptic Systems |
Cat# 226 004; RRID:AB_2619946 |
Rabbit anti-cFOS |
Abcam |
Cat# ab190289; RRID:AB_2737414 |
Mouse anti-neurofilament |
Biolegend |
Cat# SMI-312R; RRID:AB_2314906 |
Alexa goat anti-rabbit 488 |
Molecular Probes |
Cat# A11008; RRID: AB_143165 |
Alexa goat anti-chicken 488 |
Molecular Probes |
Cat# A11039; RRID: AB_142924 |
Alexa goat anti-rabbit 546 |
Molecular Probes |
Cat#A11010; RRID: AB_143156 |
Alexa goat anti-mouse 546 |
Molecular Probes |
Cat#A11003; RRID: AB_141370 |
Alexa goat anti-guinea pig 488 |
Molecular Probes |
Cat# A-11073; RRID:AB_142018 |
Alexa goat anti-guinea pig 546 |
Molecular Probes |
Cat# A-11074 RRID:AB_2534118 |
Bacterial and Virus Strains |
AAV2/5 GfaABC1D ChR2(H134R)-mCherry SV40 |
This manuscript, UPenn Vector Core |
Available upon request from UPenn |
AAV2/1 hSynapsinl ChR2(H134R)-eYFP SV40 |
UNC GTC vector core |
Lot# AV4319J |
Chemicals, Peptides, and Recombinant Proteins |
TTX |
Cayman Chemical Company |
Cat# 14964 |
CNQX |
Tocris |
Cat# 0190 |
D-AP5 |
Tocris |
Cat# 0106 |
Barium chloride |
Sigma |
Cat# 202738 |
Normal goat serum |
Vector |
Cat# S-1000 |
Critical Commercial Assays |
QIAquick PCR Purification Kit |
QIAGEN |
Cat# 28104 |
QIAGEN Mini kit |
QIAGEN |
Cat# 27104 |
QIAGEN Endofree MAXI kit |
QIAGEN |
Cat# 12362 |
QIAquick Gel extraction Kit |
QIAGEN |
Cat# 28704 |
Experimental Models: Organisms/Strains |
Mouse: C57BL/6N inbred mice |
Taconic |
JAX Stock # 005304 |
Mouse: Thy1-ChR2-YFP |
Arenkiel et al., 2007 |
JAX Stock # 007615 |
Recombinant DNA |
pZac2.1 GfaABC1D ChR2(H134R) SV40 |
This manuscript; Addgene |
Plasmid # 112496 |
Software and Algorithms |
OriginPro 8.5/9/2015 |
Origin Lab |
N/A |
GraphPad Instat 3 |
GraphPad Software |
N/A |
pCLAMP10 |
Molecular Devices |
N/A |
ClampFit 10.5 |
Molecular Devices |
N/A |
Fluoview FV3000 |
Olympus |
N/A |
ImageJ v1.30 |
ImageJ |
N/A |
ANY-maze behavioral tracking software |
Stoelting Co. |
N/A |
Prizmatix Pulser |
Prizmatix Ltd. |
N/A |
MSN model parameter file |
GSL file submitted here |
N/A |