KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-FRQ (rabbit) | Jay Dunlap (Garceau et al., 1997) | N/A |
| Anti-WC-1 (rabbit) | Jay Dunlap (Froehlich et al., 2002) | N/A |
| Anti-WC-2 (rabbit) | Jay Dunlap (Denault et al., 2001) | N/A |
| Anti-V5 (mouse) | Thermo Pierce | N/A |
| Anti-HA (rabbit) | Abcam | Ab9110 |
| Goat Anti-Rabbit IgG (H + L)-HRP Conjugate | BIO-RAD | #1706515 |
| Goat Anti-Mouse IgG (H + L)-HRP Conjugate | BIO-RAD | #1706516 |
| Bacterial and Virus Strains | ||
| E.coli, DH5alpha, Electro-competent | PROTEIN EXPRESS | 961-008 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Protease Inhibitor Cocktail | Roche | 11836170001 |
| Phos-tag reagent | ApexBio | F4002 |
| SuperSignal West Pico ECL (Pierce) | Thermo Fisher | 34078 |
| Critical Commercial Assays | ||
| CDK1-cyclin B | New England Biolabs | P6020 |
| Casein Kinase I (CK1) | New England Biolabs | P6030 |
| Casein Kinase II (CK2) | New England Biolabs | P6010S |
| p42 MAP Kinase (MAPK) | New England Biolabs | P6080 |
| Lambda Protein Phosphatase (Lambda PP) | New England Biolabs | P0753 |
| Alkaline Phosphatase, Calf Intestinal (CIP) | New England Biolabs | M0290 |
| Anti-V5 Agarose Affinity Gel antibody produced in mouse | Sigma-Aldrich | A7345 |
| SilverQuest Staining Kit | Invitrogen | LC6070 |
| Experimental Models: Organisms/Strains | ||
| S. cerevisiae: FY834 | Fungal Genetics Stock Center (Winston et al., 1995) | FGSC #9721 |
| N. crassa: WT: 328-4 | Fungal Genetics Stock Center | FGSC #1858 |
| N. crassa: WT: 661-4a (Pfrq(c-box)@his-3) A | Gooch et al., 2008 | N/A |
| N. crassa: −1-6: wc-1V5::bar+; ras-1bd | Wang et al., 2014 | −1-6 |
| N. crassa: ras-1bd; Δwc-1::hph+; Δmus-52::hph+ a | Wang et al., 2014 | 21-9 |
| N. crassa: ras-1bd; Δwc-2::hph+; Δmus-52::hph+ a | Wang et al., 2014 | 173-1 |
| N. crassa: Δpkac-1::hph+ | Fungal Genetics Stock Center (Colot et al., 2006) | FGSC#11513 |
| N. crassa: Δpkac-2::hph+ | Fungal Genetics Stock Center (Colot et al., 2006) | FGSC#11433 |
| N. crassa: rgb-1RIP | Fungal Genetics Stock Center | FGSC#8380 |
| Recombinant DNA | ||
| Plasmid: pRS426: Yeast episomal vector with URA3 marker, and Ampicillin resistance | Fungal Genetics Stock Center (Colot et al., 2006) | pRS426 |
| Software and Algorithms | ||
| NIH ImageJ with a custom macro and period lengths were calculated using custom MATLAB software | Larrondo et al., 2015 | N/A |
| ChronOSX 2.1 |