Table 1. Primers designed for qRT-PCR validation of candidate lncRNAs and mRNAs.
Name lncRNA/mRNA | Primer | Tm(ºC) | Product length(bp) |
---|---|---|---|
GAPDH(MOUSE) | F:5’ CACTGAGCAAGAGAGGCCCTAT3’ R:5’ GCAGCGAACTTTATTGATGGTATT3’ |
60 | 144 |
ENSMUST00000124572 | F:5’ GGTTAAAGCAACGGACAGAG 3’ R:5’ TGGCAAAGTCCTTACCACAG 3’ |
60 | 96 |
ENSMUST00000180989 | F:5’AACCCGACAATGTAAGGACC3’ R:5’ TGTAAAACCGCACAAGGCT3’ |
60 | 200 |
ENSMUST00000147219 | F:5’CCAGTTGAGGAGACAGGGAA3’ R:5’ TGGCTAGAGCAGGGGATTA3’ |
60 | 92 |
ENSMUST00000097928 | F:5’TTGGAATGCCTTGGAGATG3’ R:5’ GTTGGTTGTCACCGTTGCT 3’ |
60 | 145 |
uc007mos.1 | F:5’GCTGAGGCTTTGATGTTGC3’ R:5’ TCCAGTTCTTGACGCTCTTCT 3’ |
60 | 115 |
ENSMUST00000145410 | F:5’CTACCTGGACCCCAATACAAC3’ R:5’ ACCCAAGGCAAGTCACAAA3’ |
60 | 181 |
NR_040589 | F:5’GGACAGGATTTGGATTTCGA3’ R:5’ TGACAGACCACCATAACAGACA3’ |
60 | 184 |
ENSMUST00000139773 | F:5’CAGTGCTCAAGAGACTCAGAAAA3’ R:5’ AACAGGTGCTGGTCAAAGG3’ | 60 | 259 |
ENSMUST00000169128 | F:5’ CCATCTAATGCCCTTTTCTG 3’ R:5’ GCTTGTTCTGTATGTACTGGACC 3’ |
60 | 150 |
NM_025684 | F:5’ CCTCCGAGACCTGAAACATC 3’ R:5’ CCCTCCAGTGCCTTGAAAT 3’ |
60 | 279 |
AK078749 | F:5’ CGCTAATTCTTCCTCCGTG 3’ R:5’ TGATTGGTCCGCACTTCTT 3’ |
60 | 135 |
NM_028746 | F:5’ ACTCGGTGTCATTTCCCTCA 3’ R:5’ TTCTTCAGCTCCCCTGCTAT 3’ |
60 | 189 |
NM_013913 | F:5’ AACAGAGGCGAACATACAAGTG3’ R:5’ CGTTGAAGTCCTGTGAGCCAT 3’ |
60 | 139 |
NM_020013 | F:5’ GGAGGATGGAACAGTGGTAGGC3’ R:5’ AGGCTTTGACACCCAGGATTTG3’ |
60 | 104 |
NM_009114 | F:5’ ATACTGGGCTTACACTGCTCTT3’ R:5’ CTGTGCTTCCACCATTTGTC3’ |
60 | 193 |