Skip to main content
. Author manuscript; available in PMC: 2020 Feb 14.
Published in final edited form as: J Med Chem. 2019 Feb 5;62(3):1643–1656. doi: 10.1021/acs.jmedchem.8b01958

Figure 6.

Figure 6.

Concentration-dependent elevation of cytokines and chemokines in M1-polarized THP-1 macrophages treated with the ABHD12 inhibitor DO264. (A) PMA-differentiated THP-1 cells were polarized to M1 macrophages by stimulation with 20 ng/mL IFN-γ and 10 pg/mL LPS. (B) Cytokines and chemokines were measured 6 and 24 h after stimulation. DO264 and (S)-DO271 were included in the media at the specified concentrations throughout the experiment (A). Media samples from DMSO-treated resting (M0) macrophages were included as an additional control. Data represent mean ± SEM from four independent biological experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 (Student’s t-test performed relative to M1-DMSO samples).