Table 1. Sequence for forward and reverse primer sets used for real-time PCR.
Amplification | Forward primer | Reverse primer |
---|---|---|
LOX | 5’-CGACCCTTACAACCCCTACA-3’ | 5’-AAGTAGCCAGTGCCGTATCC-3’ |
α-SMA | 5′ -GACAATGGCTCTGGGCTCTGTAA-3′ | 5′ -CTGTGCTTCGTCACCCACGTA-3′ |
collagen1A | 5′ -GGAATGAAGGGACACAGAGG-3′ | 5′ -TAGCACCATCATTTCCACGA-3′ |
CTGF | 5′ -CTCCTGCAGGCTAGAGAAGC-3′ | 5′ -GATGCACTTTTTGCCCTTCTT-3′ |
fibronectin | 5′ -CTGGCCGAAAATACATTGTAA-3′ | 5′ -CCACAGTCGGGTCAGGAG-3′ |
RhoA | 5’ -CGTTAGTCCACGGTCTGGTC-3’ | 5’-GCCATTGCTCAGGCAACGAA-3’ |
PDGF-B | 5’ -TGATCTCCAACGCCTGCT-3’ | 5’-TCATGTTCAGGTCCAACTCG-3’ |
MCP-1 | 5’ -CTGAAGCTCGTACTCTC-3’ | 5’-CTTGGGTTGTGGAGTGAG-3’ |
β-actin | 5′ -GTCCACCTTCCAGCAGATGT-3′ | 5′ -AAAGCCATGCCAATCTCATC-3′ |
LOX: lysyl oxidase; α -SMA: smooth muscle actin; CTGF: connective tissue growth factor; TGF: transforming growth factor; PDGF: platelet derived growth factor; MCP: Monocyte chemoattractant protein.