Table 1. Summary of the purification steps of AaeLOX4 in E. coli.
Step | Total protein (mg) |
Specific activity (U · mg-1) |
Purification (fold) |
---|---|---|---|
Crude extract | 146.97 | 0.038 | 1.0 |
Affinity chromatography | 37.16 | 0.329 | 8.6 |
Gel filtration | 0.09 | 51.34 | 1351.1 |