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. Author manuscript; available in PMC: 2019 Jun 19.
Published in final edited form as: ACS Synth Biol. 2019 Jan 29;8(2):403–414. doi: 10.1021/acssynbio.8b00430

Table 1.

Outline of Protocol Differences between Extract Preparation Methodsa

extract protocol Zubay, 19734 Sun et al., 201359 Kwon and Jewett, 201558 this work
starter culture one 16-h culture two 8-h cultures one 16-h culture one 16-h culture
culture media composition 2× YT 2× YT + phosphate 2× YT + phosphate + glucose 2× YT + phosphate
lysis method French press bead-beating sonication sonication
postlysis centrifugation 30 000g for 30 min 12 000g for 10 min 12 000g for 10 min 12 000g for 10 min
run-off reaction 80min 80 min strain-dependent; none for BL21 80 min
dialysis 18 h 3 h none 3 h
flash freeze none none before lysis optional
a

Italicized: variables examined in this study.