Table 1.

Number of Contacts^a

Frequency cutoff	Hybrid H2A-H2B	Hybrid macroH2A-H2B	Inter- dimer	canonical dimer 1	canonical dimer 2	Inter- dimer	macroH2A dimer1	macroH2A dimer2	Inter- dimer
0.6	19	19	3	22	19	1	17	20	0
0.65	17	15	3	18	18	1	16	16	0
0.7	17	12	3	16	16	1	14	15	0
0.75	17	12	2	15	13	0	13	10	0
0.8	16	11	1	13	11	0	10	9	0

^aObserved between each (macro)H2A−H2B dimer and the core tetramer, as well as between dimer moieties, in each simulation using several frequency cutoffs and distance requirement of 3.5 Å between heavy atoms in each residue-pair contact. Canonical dimers in the hybrid simulations possess more contacts with the tetramer with increasing stringency of the frequency cutoff. To a slightly lesser extent, the same trend is seen between identically positioned moieties in the homogeneous systems. Contacts between the hybrid canonical dimer are also observed to be more robust than the identically placed dimer in the canonical simulations, and the macroH2A moiety displays the same effect. At every frequency cutoff, the hybrid system possesses the largest number of inter-dimer contacts.