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Hydroxyproline (HOP) content in the hearts of AAV-S15D-RLC or PBS injected D166V and WT mice and non-injected controls was measured in female and male mice. 5-13 heart samples from 7-8 D166V and 3-6 WT animals were used. All assays were performed in triplicate. Data were plotted as mean ± SEM (n= number of samples). Comparisons among D166V and WT mice were performed using two-way ANOVA with Tukey’s multiple test with *P<0.05, ***P<0.001.
