Table 1.
Assessment of intact heart function in AAV vs PBS-injected D166V HCM and WT-RLC mice.
| Echocardiography | ||||
|---|---|---|---|---|
| D166V AAV | D166V PBS | WT AAV | WT PBS | |
| No. of animals (n) | 6F, 6M | 5F, 3M | 3F, 3M | 3F, 3M |
| Heart/body (mg/g) | 4.25±0.16 | 4.58±0.30 | 5.01±0.43 | 4.56±0.65 |
| HR (bpm) | 490±14 | 504±31 | 452±20 | 424±30 |
| EF (%) | 71±2 | 65±4 | 62±4 | 63±4 |
| FS (%) | 51±3 | 49±5 | 41±3 | 38±2 |
| LVAW;d (mm) | 1.06±0.04** | 1.41±0.13 | 0.98±0.08 | 1.10±0.05 |
| LVAW;s (mm) | 1.78±0.09 | 2.04±0.16 | 1.57±0.09 | 1.65±0.05 |
| LVID;d (mm) | 3.64±0.14* | 3.10±0.18 | 3.62±0.11 | 3.78±0.16 |
| LVID;s (mm) | 1.80±0.16 | 1.60±0.23 | 2.15±0.16 | 2.37±0.17 |
| LVPW;d (mm) | 0.96±0.04* | 1.42±0.22 | 0.98±0.15 | 1.04±0.07 |
| LVPW;s (mm) | 1.60±0.09* | 2.01±0.18 | 1.37±0.17 | 1.50±0.10 |
| LV mass/corr (mg) | 112.7±6.66 | 157.60±25.57 | 110.7±18.02 | 129.8±12.04 |
| Doppler Analysis | ||||
| IVCT (ms) | 13.61±1.16* | 19.22±2.05 | 15.14±1.10* | 21.84±2.53 |
| IVRT (ms) | 20.82±1.25 | 19.59±2.62 | 15.90±1.54 | 15.66±1.25 |
| MV A (mm/s) | 533±44 | 447±54 | 345±39 | 318±29 |
| MV E (mm/s) | 810±44** | 547±82 | 655±34 | 645±46 |
| MV E/A | 1.36±0.06 | 1.35±0.14 | 1.89±0.15 | 1.98±0.16 |
| MPI | 0.59±0.03* | 0.69±0.03 | 0.66±0.05 | 0.80±0.06 |
| Invasive Hemodynamics | ||||
| SW (mmHg*μl) | 2261±211*** | 918±211 | 2892±151 | 2448±445 |
| CO (μl min−1) | 15686±1383*** | 6653±1323 | 15243±1443 | 14006±2897 |
| SV (μl) | 31.27±2.48* | 18.85±4.71 | 33.67±1.43 | 32.76±6.57 |
| ESV (μl) | 22.50±2.58 | 19.23±3.09 | 22.48±4.78 | 24.67±6.15 |
| EDV (μl) | 49.16±4.17* | 35.70±3.59 | 53.80±3.25 | 53.92±11.02 |
| Pes (mmHg) | 86.08±2.98* | 73.65±5.59 | 89.29±6.85 | 78.42±6.16 |
| Ped (mmHg) | 6.42±0.89 | 13.15±3.92 | 7.42±1.04 | 7.33±0.87 |
| HR (bpm) | 501±14 | 459±22 | 422±23 | 393±21 |
| Ea (mmHg μl−1) | 2.97±0.29* | 5.34±1.00* | 2.61±0.40 | 3.05±1.00 |
| dP/dtmax (mmHg s−1) | 8409±473 | 7441±998 | 6988±370 | 6426±663 |
| −dP/dtmin (mmHg s−1) | 5104±292 | 4961±729 | 7307±527 | 5825±595 |
| Tau (ms) | 9.93±0.66** | 13.68±0.85* | 7.89±0.37 | 7.77±0.59 |
| ESPVR (mmHg μl−1) | 2.56±0.45 | 2.42±0.48 | 2.29±0.41 | 2.74±0.34 |
| EDPVR (mmHg μl−1) | 0.13±0.03 | 0.29±0.09 | 0.16±0.06 | 0.11±0.02 |
| PRSW (mmHg) | 56.04±4.57* | 36.36±5.85 | 55.15±5.77 | 58.50±3.92 |
| dP/dtmax-EDV (mmHg s−1 μl−1) | 110.7±11.75* | 60.93±17.49 | 60.20±7.09* | 113.5±16.00 |
| Strain Analysis | ||||
| COstr (μl min−1) | 13344±1218*** | 5250±543 | 12217±1583 | 13000±1192 |
| EFstr (%) | 79±3*** | 56±4 | 62±5 | 62±2 |
| FSstr (%) | 49±4** | 33±4 | 31±4 | 32±2 |
| EDVstr (μl) | 35.24±2.82* | 24.10±3.02 | 48.13±8.14 | 46.68±3.74 |
| SVstr (μl) | 27.83±2.32** | 13.93±2.65 | 29.10±4.07 | 29.07±2.45 |
| GLS (%) | −26.06±0.84**** | −10.92±2.91 | −20.63±2.05 | −22.67±1.66 |
Data are mean ± SEM with *P<0.05, **P<0.01, ***P<0.001, and ****P<0.0001 depicting significance between AAV vs PBS treated mice, by Student’s t-test.
Abbreviations: Heart weight/body weight in mg/g; Heart weigh/tibia length in mg/mm; HR, heart rate in beats per minute; EF%, ejection fraction; FS%, fraction of shortening; LVAW, left ventricular anterior wall; LVID, left ventricular inner diameter; LVPW, left ventricular posterior wall; d, diastolic; s, systolic; IVRT, isovolumetric relaxation time; IVCT, isovolumetric contraction time; MV E/A, mitral valve inflow -peak E-wave to A-wave velocity ratio; E, transmitral early peak velocity; E’, early diastolic mitral annulus velocity; MPI, Myocardial Performance Index; CO, cardiac output; SW, stroke work; SV, stroke volume; ESV, end-systolic volume; EDV, end-diastolic volume; Pes, end-systolic pressure; Ped, end-diastolic pressure; Ea, arterial elastance; dP/dt max, peak rate for pressure rise; −dP/dtmin, peak rate for pressure decline; Tau, relaxation time constant; ESPVR, end-systolic PV relation (slope); EDPVR, end-diastolic PV relation (slope); PRSW, preload recruitable power stroke work; dP/dtmax-EDV, peak rate of rise in LV pressure-end diastolic volmne relationship; COstr, Cardiac Output-strain; EFstr, Ejection Fraction-strain; FSstr, Fractional Shortening-strain; EDVstr, End Diastolic Volume-strain; SVstr, Stroke Volume-strain; GLS, global longitudinal strain.