Skip to main content
. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Curr Opin Chem Biol. 2019 Mar 18;50:29–36. doi: 10.1016/j.cbpa.2019.02.010

Figure 1:

Figure 1:

(A) General isoTOP-ABPP workflow. Reactive cysteine residues on two proteome samples are labeled with IA-alkyne, followed by CuAAC with an isotopically heavy or light biotin-azide cleavable linker. The two lysates are combined, biotinylated proteins are enriched on streptavidin-agarose beads, and subjected to an on-bead trypsin digestion. The IA-labeled peptides are released and analyzed by LC/LC-MS/MS. Heavy and light peptide pairs are quantified by their extracted MS1 peaks. (B) IA-alkyne structure and cysteine-labeling scheme. (C) The tobacco-etch virus (TEV) protease cleavable biotin-azide tag for isoTOP-ABPP.