Production of antibodies against hFSH is associated with poor second ovarian response. A: Detection of antibodies against hFSH in serum. Because the level of antibodies against hFSH
from first to second GnRH administrations was 1 ± 0.46, we considered that the antibody level was elevated significantly when the level of antibodies against hFSH exceeded 1.46. Red
and black lines indicate Ab (+) and Ab (–), respectively. B: Numbers of total oocytes collected from cynomolgus monkeys without or with the antibody production. C: Percentages of MII
oocytes collected from cynomolgus monkeys without or with the antibody production. D: Percentages of GV, MI, MII, and DG oocytes collected from cynomolgus monkeys without or with the
antibody production at the second superovulation. n.s; not significant.