Table 1. Effect of manganese treatment on nuclear maturation of porcine oocytes during IVM.
| Manganese concentration (ng/ml) |
No. of oocytes cultured 1 |
No. (mean ± SEM, %) of oocytes at the stage of |
|||
|---|---|---|---|---|---|
| GV-GVBD 2 | MI 3 | Ana-Telo 4 | MII 5 | ||
| 0 | 180 | 0 (0.0 ± 0.0) | 9 (5.0 ± 1.1) | 10 (5.5 ± 1.1) | 161 (89.4 ± 2.0) |
| 3 | 180 | 1 (0.1 ± 0.1) | 8 (5.0 ± 1.1) | 7 (3.8 ± 1.0) | 164 (91.1 ± 1.4) |
| 6 | 180 | 0 (0.0 ± 0.0) | 10 (5.5 ± 0.7) | 5 (2.7 ± 1.3) | 165 (91.6 ± 1.6) |
| 12 | 180 | 0 (0.0 ± 0.0) | 6 (3.3 ± 1.7) | 6 (3.3 ± 0.8) | 168 (93.3 ± 2.2) |
1 Experiment was replicated 6 times. 2 GV-GVBD, Germinal vesicle-Germinal vesicle breakdown. 3 MI, Metaphase I. 4 Ana-Telo, Anaphase-Telophase I. 5 MII, Metaphase II.