Table 2. Developmental competence of cat embryos after Piezo-ICSI using testicular sperm and elongated spermatids (experiment 2).
Treatment | Number of oocytes | Stage of development (%) |
Blast cells, N | |||
---|---|---|---|---|---|---|
Cleavage | Morula | Blastocyst | ||||
Testicular sperm | Activation | 113 | 77.8 ± 3.2 a | 20.5 ± 2.3 a, b | 11.3 ± 4.3 | 132.8 ± 8.7 |
No activation | 123 | 50.2 ± 4.1 b | 10.0 ± 2.1b | 7.6 ± 2.0 | 151.8 ± 26.3 | |
Elongated spermatid | Activation | 123 | 71.8 ± 3.7 a | 22.2 ± 2.7 a | 4.1 ± 2.5 | 151.0 ± 33.2 |
No activation | 122 | 43.7 ± 5.5 b | 9.0 ± 4.2 b | 4.4 ± 1.4 | 117.8 ± 13.8 | |
Sperm | 154 | 50.5 ± 6.7 b | 14.0 ± 2.9 a, b | 12.2 ± 2.8 | 151.8 ± 20.8 |
Date are expressed as the mean ± the SEM. Different superscripted letters indicate significant differences (P < 0.05).