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. 2019 May;10(3-4):63–79. doi: 10.18632/genesandcancer.191

Figure 5. Transcriptional activity of ABC/β-catenin increases with OS progression.

Figure 5

mRNA expression of Wnt/β-catenin target genes (MMP2, MMP9, Cyclin D1, VEGF-A and Axin2 in: A. SaOS2 and SaOS2-LM7 cell lines and B. HOS and HOS-143B cell lines. Target gene expression was quantified by RT-qPCR and shows that mRNA expression of MMP2, MMP9, Cyclin D1, VEGF-A was higher in the SaOS2-LM7 and HOS-143B cell lines compared to the parental SaOS2 or HOS cells, respectively. On the other hand, mRNA expression of Axin2 was significantly lower in the SaOS2-LM7 and HOS-143B cell lines compared to the parental SaOS2 or HOS cells, respectively. Each group of data is representative of 4 or more experiments. *p < 0.05; ***p < 0.0005. C. -catenin/TCF transcriptional activity as measured by TopFlash reporter assay were higher in the HOS-143B and SaOS2-LM7 cells compared to parental HOS and SaOS2 cells, respectively. Each group of data is representative of 6 or more experiments. *p < 0.001; **p < 0.0001.