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. 2019 May;10(3-4):80–96. doi: 10.18632/genesandcancer.192

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Copyright: © 2019 Al-Jameel et al.

This article is distributed under the terms of the Creative Commons Attribution License (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.

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A. Representative histograms for unstained PNT2, LNCaP, 22RVI and PC3-M cells without adding BODIPY-labelled fatty acid. The marker M1 highlights negative peaks of the subclass control. B. Representative histograms for fluorescence of stained PNT2, LNCaP, 22RVI and PC3-M cells 30 min after adding BODIPY-fluorescently labelled fatty acid; the marker M2 is placed to the right of M1 to highlight positive events (total percentage of cells with BODIPY-labelled fatty acid). C. Percentages of cells taking up BODIPY-labelled fatty acid from different prostate epithelial cell lines. D. Representative histograms for fatty acid uptake of PC3-M cells at a fixed concentration of BODIPY-labelled fatty acid in the presence of different concentrations of dmrFABP5. M1, unstained cells; M2, stained cells. E. Percentages of cells with fluorescently-labelled fatty acid in PC3-M control (untreated) and those wells treated with different concentrations of dmrFABP5 for 30 min with a fixed concentration of BODIPY-labelled fatty acid. Fluorescent intensity of each cell line was measured with an EPICS XL Cytometer (Beckman) at 570nm and data analysis was performed with SYSTEM II^™ Software. Values were plotted as mean ± SE (error bars). The differences between the control and the experimental groups were assessed by 2-tailed unpaired Student's t test. ^*, p < 0.01; ^**, p < 0.001.