Fig. 5.
The effect of anti-PD-L1 together with CT on mice harboring large Hepa1-6 tumors. a, b C57BL/6 mice (female, 8 weeks-old, n = 5) were inoculated s.c. with 2 × 106/mouse of Hepa1-6 cells in the right flank on day 1 and the formation of tumors was monitored. When tumors reached approximately 1.0 cm in diameter (day 9 ~ 10), tumor-bearing mice were treated with i.t. injection of CT (100 μg/mouse) every other day or CT combined with i.t. injection of anti-PD-L1 antibody (10 μg/mouse) twice weekly for 2 weeks. Tumor growth (mean ± SD) and survival were monitored and plotted. Shown are the results of one experiment representative of three. *p < 0.05 and **p < 0.001. c, d The mice cured of hepatoma by the treatment with CT and anti-PD-L1 were inoculated s.c. with 2 × 106/mouse of Hepa1-6 cells in the right flank and 2 × 105/mouse of EG7 cells in the left flank. The growth of tumors on both flanks was monitored and graphed