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Phenotypic expression of tight junction proteins in μHuB. The hCMEC/D3 monolayers in μHuB express tight junction proteins (ZO‐1, green & Claudin‐5, red) under (a) static culture for 3 days and (b) when conditioned to physiologically relevant flow (2.73 dyn/cm2) using a linear ramp conditioning overnight protocol. Expression of both ZO‐1 and Claudin‐5 increased in response to the fluid flow. (scale bars = 200 μm)
