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. 2019 Jan 13;4(2):e10126. doi: 10.1002/btm2.10126

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© 2018 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals, Inc. on behalf of The American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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hCMEC/D3 monolayers remain viable during dynamic culture before and after analyte transport. hCMEC/D3 monolayers in μHuB remain metabolically active as demonstrated by high levels of red, C₁₂‐resazurin (alive) fluorescence with negligible expression of green, SYTOX fluorescence (injured) after (a) static culture for 3 days (b) after conditioning monolayers overnight using the linear ramping protocol to 2.73 dyn/cm² and (c) after conducting a transport experiment using nonfluorescent dextran 70 kDa. (scale bars = 200 μm)