Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 19;5(6):eaav9462. doi: 10.1126/sciadv.aav9462

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 4 — (A) Persister cells filamentation does not solely rely on the SOS response. Fluorescence microscopy snapshots of persister filaments of the wild-type strain (left), the SOS OFF mutant (middle), and the ΔSulA mutant (right) during recovery in microfluidic time-lapse experiments are shown. The different strains contain the psulA::gfp reporter plasmid. Persister filaments are indicated by a red arrow. (B) Persister cells form polynucleated filaments during recovery. Fluorescent microscopy snapshots of the MG1655 strain containing the hupA::gfp reporter during time-lapse experiments in the microfluidic chamber are shown. The red arrow indicates a persister cell during the growth and treatment phases. White arrows indicate DNA-free regions separating well-segregated nucleoids within the filament. A DNA-free pole of the persister filament is boxed in red. Time at which pictures were taken is indicated at the bottom-left corner of each image. (C) Persister filament maximal area is slightly decreased in a ΔSulA mutant during recovery. The maximal area (μm²) of persister filaments was monitored for the MG1655 wild-type strain and the isogenic ΔSulA mutant. Bar graphs represent mean values, and values for all persister cells are plotted as dots. A Student’s t-test was performed between data for the wild-type strain and the ΔSulA mutant (*P < 0.05). (D) Persister cells do not contain reduced levels of DNA before the ofloxacin treatment. Fluorescence of the MG1655 wild-type strain containing the HU-GFP reporter plasmid is represented for persister (bars) and nonpersister cells (black dots) within a same microcolony immediately before ofloxacin treatment during time-lapse microscopy in the microfluidic chamber. The number of cells within every microcolony is n = 25 (p1_HU), n = 31 (p2_HU), n = 24 (p3_HU), n = 38 (p4_HU), n = 188 (p5_HU), and n = 52 (p6_HU).