Figure 2.

Dual-luciferase reporter assay with constructs containing BARD1 promoter. (A) Schematic of reporter gene constructs having the BARD1 promoter, with different haplotypes containing three SNPs in BARD1 5'-UTR (rs34732883, rs17489363 and 1129804). 293T cells (B) and SH-SY5Y (C) were co-transfected with 50 ng of the reporter plasmid and 10 ng of Renilla luciferase reporter plasmid for standardization of the transfection efficiencies. The luciferase activity was expressed as Mean ± S.E. of the ratio of firefly expression of BARD1 promoters. (*) indicate P < 0.05 with respect to the general (without stimulation). Abbreviation: NC, negative control.