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. 2019 May 21;10(10):2261–2275. doi: 10.7150/jca.31077

Figure 4.

Figure 4

Knockdown of SNORA71A inhibited cell proliferation and induced cell cycle arrest via the MAPK/ERK pathway. (A&B) qRT-PCR analysis indicated that knockdown of SNORA71A had no effect on its host gene SNHG17 expression, and MTT assays indicated that knockdown of SNORA71A reduced cell proliferation in both A549 and PC9 cells. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (C) Representative images of Ki-67 staining in A549 and PC9 cells. Ki67 was downregulated after knockdown of SNORA71A. The images were taken at 400X magnification. The scale bar was 20 μm. (D) Colony formation assay indicated that knockdown of SNORA71A reduced colonies numbers 12 days after transfection. (E&F) Flow cytometry was performed to detect the percentage of cells at different cell-cycle phases after propidium iodide staining. The data were shown here as the mean ± S.D. from 3 independent experiments. *, p < 0.05; **, p < 0.01. (G&H) Western blot analysis of the cell cycle-related proteins, cyclin D1, cyclin E1, CDK2/4/6, P27 and classical MAPE/ERK pathway proteins, t-ERK1/2, p-ERK1/2, t-MEK, p-MEK. GAPDH was used as an internal control.