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. 2019 May 21;10(10):2261–2275. doi: 10.7150/jca.31077

Figure 5.

Figure 5

Knockdown of SNORA71A inhibited cell migration and invasion. (A) Wound healing assay indicated that knockdown of SNORA71A decreased wound closure 48 h after scratch in both A549 and PC9 cells. The images were taken at 100X magnification. The scale bar was 200 μm. The data were shown here as the mean ± S.D. from 3 independent experiments. ***, p < 0.001. (B&C) Transwell assays indicated that knockdown of SNORA71A reduced migrative and invasive cell numbers. The images were taken at 100X magnification. The scale bar was 200 μm. The data were shown here as the mean ± S.D. from 3 independent experiments. **, p < 0.01; ***, p < 0.001. (D) Representative images of ZO-1 staining in A549 and PC9 cells. ZO-1 was upregulated in A549 and translocated to the cell membrane surface in PC9 after SNORA71A deficiency, marked by the green arrows. The images were taken at 400X magnification. The scale bar was 20 μm. (E) Invasion-related proteins, N-Cadherin, E-Cadherin, Vimentin, MMP9 and MMP2 were detected by Western blot analysis. GAPDH was used as an internal control.