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. 2019 May 21;10(10):2176–2184. doi: 10.7150/jca.32731

Figure 2.

Figure 2

Knockdown of UBE2C suppressed cell growth via inhibiting ERK/AKT signaling pathways. (A) qRT‑PCR analysis of UBE2C mRNA levels in A375 and SK-MEL-28 cells following lentiviral shNC or shUBE2C infection. Data are expressed as mean ± SD of 3 independent experiments. ***P<0.001. (B) Western blot analysis of UBE2C protein levels in A375 and SK-MEL-28 cells following lentiviral shNC or shUBE2C infection. GAPDH was used as the loading control. (C) WST-1 assays were performed to determine cell viability in A375 and SK-MEL-28 cells after lentiviral infection of shNC or shUBE2C. **P<0.01, ***P<0.001. (D-E) Western blot analysis of key protein levels in ERK/AKT signaling pathways after lentiviral infection of shNC and shUBE2C in A375 and SK-MEL-28 cells, respectively. GAPDH was used as the loading control.