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. 2019 May 21;10(10):2176–2184. doi: 10.7150/jca.32731

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Knockdown of UBE2C led to G2/M arrest via inhibiting MPF in melanoma cells. (A-B) Flow cytometry with PI staining was used to analyze the cell cycle distribution of A375 and SK-MEL-28 cells following shNC or shUBE2C infection. (C-D) Statistical analysis of cell percentage in G0/G1, S, G2/M in A375 and SK-MEL-28 cells with shNC or shUBE2C infection, Data are expressed as mean ± SD of 3 independent experiments. **P<0.01, ***P<0.001. (E-F) Expression levels of MPF complex, composed of cyclin B1 and CDK1, as well as the potential regulators of MPF activity were determined by western blot in A375 and SK-MEL-28. GAPDH was used as the loading control. (G) CDK2 and Cyclin E were detected in A375 cells after infected with shNC, and mRNA level of cyclinB1 was determined by qRT-PCR. (I) The protein level of cyclinB1 was determined after UBE2C-depletion with and without 10uM MG132 treatment for 4 hours by western blot.