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Intracellular accumulation of DOX analyzed by confocal microscope and flow cytometry. (a, c). Confocal microscope images of MDA-MB-231 cells treated with DOX, CYC + DOX, and BSA-CYC-DOX NPs for 24 h (a: DOX concentration was 1 μmol/L. c: DOX concentration was 2 μmol/L. Scale = 50 μm). (b, d). The mean fluorescence intensity of MDA-MB-231 cells treated with DOX, CYC + DOX, and BSA-CYC-DOX NPs for 24 h. (b: DOX concentration was 1 μmol/L. d: DOX concentration was 2 μmol/L).
