Table 2.
Effects of diet and timing of food intake on daily rhythms in clock and metabolic gene expression in BAT. Data was analyzed by JTK Cycle. The acrophase (in ZT) is only given for genes that are rhythmically expressed (p < 0.05). NR = non-rhythmic.
|
JTK cycle analysis for BAT |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genes BAT |
CHOW |
HFHS |
||||||||||
| Clock | Acrophase |
P-value |
Acrophase |
P-value |
||||||||
| Ad lib | D | L | Ad lib | D | L | Ad lib | D | L | Ad lib | D | L | |
| Bmal1 | 22.5 | 0 | 4.5 | 0.001 | 0.001 | 0.001 | 1.5 | 1.5 | 9 | 0.001 | 0.001 | 0.001 |
| Per2 | 13.5 | 16.5 | 21 | 0.001 | 0.001 | 0.001 | 16.5 | 16.5 | 0 | 0.001 | 0.001 | 0.001 |
| Rev-erbα | 9 | NR | NR | 0.001 | 1 | 0.092 | 10.5 | 10.5 | NR | 0.001 | 0.001 | 0.133 |
| Cry1 | 21 | 21 | 0 | 0.001 | 0.001 | 0.005 | 21 | 22.5 | 7.5 | 0.001 | 0.001 | 0.005 |
| Cry2 | 12 | 12 | 21 | 0.001 | 0.034 | 0.001 | 16.5 | 13.5 | NR | 0.001 | 0.001 | 1 |
| DBP | 12 | 12 | 19.5 | 0.001 | 0.001 | 0.001 | 13.5 | 13.5 | 21 | 0.001 | 0.001 | 0.001 |
| Metabolic | ||||||||||||
| Srebp-1c | 10.5 | NR | NR | 0.024 | 1 | 0.071 | NR | 13.5 | NR | 0.090 | 0.013 | 1 |
| Glut4 | NR | NR | NR | 1 | 0.432 | 0.189 | NR | 3 | NR | 1 | 0.007 | 1 |
| Ucp1 | NR | NR | NR | 1 | 1 | 1 | NR | 7.5 | NR | 0.736 | 0.023 | 0.736 |
| Pgc1α | 9 | 10.5 | NR | 0.001 | 0.017 | 0.117 | NR | NR | NR | 1 | 0.083 | 0.844 |
| Pparα | NR | 10.5 | NR | 1 | 0.016 | 1 | NR | 12 | NR | 0.429 | 0.004 | 0.376 |
| Fas | NR | NR | NR | 0.950 | 1 | 1 | NR | NR | NR | 1 | 1 | 1 |
| LPL | NR | NR | 0 | 0.264 | 0.07 | 0.002 | NR | NR | NR | 1 | 1 | 0.488 |
| HSL | 9 | NR | 22.5 | 0.036 | 0.253 | 0.001 | NR | NR | NR | 1 | 1 | 0.736 |