Fig. 5.

Selection of Prx3 particles and structural analysis. a, b Particles selected on a representative micrograph (micrograph number 19.22.14) of the EMPIAR-10050 dataset using either a crYOLO or b EMAN2. Scale bar, 100 nm. c Summary of particle selection and structural analysis. The resolution in parentheses is the result obtained after a 3-D refinement performed in SPHIRE using the final 8562 particles of the original dataset. d Representative 2-D class averages obtained from two rounds of classification using the crYOLO-selected particles and ISAC. Scale bar, 10 nm. Well-centered examples for all views showing high-resolution details can be readily obtained from the data. e Fourier shell correlation plots for the final 3-D reconstruction (black) using the crYOLO-selected particles or the 8562 particles from the original dataset (gray). The average resolution of our 3-D reconstruction is ~4.6 Å, whereas that one from the originally used particles is ~4.5 Å. f Top and side views of the 3-D reconstruction obtained with crYOLO/SPHIRE. For clarity, all subunits are colored differently in the reconstruction