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. 2019 Jun 19;9:8762. doi: 10.1038/s41598-019-44853-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Experimental design. On day 11 post allergen challenge, BAL was performed to determine eosinophilic inflammation. (B) Total cells from airways were counted to determine differential immune responses between the control and HDM-challenged groups. (C) Number of eosinophils assessed using cytospin followed by Diff-Quik staining. (D) Number of allergen-specific T cells was determined by flow cytometry. Data are means of three independents experiments (3/group). Values are mean ± standard error of the mean (SEM) (*P < 0.05).