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. 2019 May 6;23(7):4545–4558. doi: 10.1111/jcmm.14350

Figure 4.

Figure 4

TIGAR is involved in enhanced DSB formation in TFAM knockdown cells. A, Western blotting analysis of TIGAR expression in TFAM knockdown MCF‐7, U‐2 OS and Hep G2 cells; B, Western blotting analysis of TIGAR levels in control and TFAM knockdown U‐2 OS and Hep G2 cells after transfection with TIGAR si‐RNA or TIGAR overexpression plasmid; C, Fluorescence microscopic analysis of mitochondrial superoxide levels in control and TFAM knockdown U‐2 OS and Hep G2 cells; (D, E) The DNA double‐strand breaks levels in irradiated control and TFAM knockdown U‐2 OS and Hep G2 cells after transfection with TIGAR si‐RNA or TIGAR overexpression plasmid respectively. *, ** and *** represents P < 0.05, 0.01 and 0.001 respectively