Figure 4.

Enhancer of zeste homolog 2 (EZH2) was modulated by miR‐124‐3p and miR‐506‐3p in sorafenib resistant cells. A, The expression levels of miR‐124‐3p and miR‐506‐3p were decreased in TC‐13 cells compared with the TC‐07 cells. The mRNA levels of miR‐124‐3p and miR‐506‐3p were determined by a qRT‐PCR. The results represent the mean ± SD from three independent experiments. ***P < 0.001. B, The protein levels of EZH2 and H3K27me3 was up‐regulated, whereas, the H3K27Ac was down‐regulated in TC‐13 cells compared with the TC‐07 cells. A Western blot of cell lysates of TC‐13 and TC‐07 cell was performed to examine the protein levels of EZH2, H3K27me3 and H3K27Ac. H3 was used as the loading control. C, The relative mRNA levels of EZH2 and H3K27me3 were increased and H3K27Ac was decreased in the TC‐13 cells compared with the TC‐07 cells. The mRNA levels of EZH2, H3K27me3 and H3K27Ac were tested by a qRT‐PCR. The results represent the mean ± SD from three independent experiments. ***P < 0.001, **P < 0.05