Figure 5.

Targeting enhancer of zeste homolog 2 by overexpression of miR‐124/506 or EPZ‐6438 treatment inhibits the proliferation ability of sorafenib resistant thyroid tumor cells via epigenetic regulation. A, The proliferation ability of TC‐13 cells was inhibited when cells were overexpressed with miR‐124/506 or treated with 1 μmol/L EPZ‐6438. The cell viability was examined for 5 d by CCK‐8 kit and the absorbance results at 450 nm were represented as mean ± SD from three independent repeats. B, Overexpression of miR‐124/506 or EPZ‐6438 treatment promotes the protein expression of H3K27me3 and suppresses the protein level of H3K27Ac in thyroid tumour cells. A Western blot was performed to determine the protein levels of H3K27me3 and H3K27Ac with indicated antibodies. H3 was tested as a loading control