Figure 2.

Hyperglycaemia caused enhanced expression of HIF‐1α, SP1 and ROBO4 and down‐regulation of miR‐125b‐5p in ARPE‐19 cells. A, HIF‐1α, B, SP1 and C, ROBO4 mRNA levels under hyperglycaemic conditions compared with the normal glucose (NG) group. There were no significant changes in the mannitol (MN) group. Expression was expressed as a ratio to HPRT‐1, normalized to NG and calculated by the 2^−ΔΔCt method. D‐I, Protein levels of HIF‐1α, SP1 and ROBO4 were determined by Western blotting analysis, and the densitometric analysis was normalized to β‐actin expression. J, Determination of miR‐125b‐5p expression in ARPE‐19 cells cultured in high glucose (HG) for 5 days using RT‐qPCR, normalized to RNU6B. All groups, n = 3; *P < 0.05; **P < 0.01; ***P < 0.001 versus the respective NG