Figure 1.

Relative Lnc‐NA and nuclear receptor subfamily 4 group A member 1 (NR4A1) expression in Endometrioid endometrial carcinoma (EEC) tissues and EEC cell lines. (A) Lnc‐NA expression levels in normal adjacent endometrial tissues (n = 30) and EEC tissues (n = 30) were examined by qRT‐PCR and normalized to GAPDH expression. (B) NR4A1 expression levels in normal adjacent endometrial tissues (n = 30) and EEC tissues (n = 30) were examined by qRT‐PCR and normalized to GAPDH expression. (C) Lnc‐NA and NR4A1 mRNA levels were positively associated in 30 EEC tissues (r = 0.454, P = 0.0191). (D) Lnc‐NA expression levels in EEC cell lines (n = 4) and normal endometrial cells were detected by qRT‐PCR. (E) NR4A1 expression levels in EEC cell lines (n = 4) were detected by Western blot. (F) Grey value analysis of the Western blot results. (G) Lasergene software was used to predict the region of NR4A1 that is anti‐sense to Lnc‐NA. (H) Luciferase activity assays in 293T cells showed the enhancement of NR4A1 promoter activity by Lnc‐NA (*P < 0.05). All data are shown as the means ± SD. Significant differences between groups are indicated as *P < 0.05; **P < 0.01; and *** P < 0.001