Figure 4.

Nuclear receptor subfamily 4 group A member 1 (NR4A1) is a target of Lnc‐NA. (A) The effects of NR4A1 on cell proliferation after NR4A1 knockdown in Ishikawa‐Lnc‐NA cells were evaluated using the CCK‐8 assay. (B) The effects of NR4A1 on cell apoptosis after NR4A1 knockdown in Ishikawa‐Lnc‐NA cells were evaluated using FACS. (C) The effects of NR4A1 on migration and invasion in Ishikawa‐Lnc‐NA cells were determined using migration and invasion assays. (D) Western blots show NR4A1, Bax, Bcl2, MMP2/9 protein expression levels when NR4A1 was knocked down in Ishikawa‐Lnc‐NA cells. All data are shown as the means ± SD, n = 3. Significant differences between groups are indicated as **P < 0.01, and ***P < 0.001